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KMID : 1161520010050030263
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Animal Cells and Systems
2001 Volume.5 No. 3 p.263 ~ p.266
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Metallothionein Induction in Liver Regeneration Stimulated by Partial He patectomy
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Kim Wan-Jong
Shin Kil-Sang
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Abstract
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Metallothionein (MT) is induced in the regenerating rat liver. We have investigated expression of MT gene by RT PCR as well as specific localization of MT by immunocytochemistry in regenerating rat liver after partial hepatectomy (PH). MT mRNA level started to increase from 1h and reached the peak at 8 h after PH. The level decreased gradually by 24 h, and became similar to that of control group. In the immunocytochemical study, in all groups treated with primary antibody, immunogold particles indicating the presence of MT were evenly distributed throughout both cytoplasm and nucleus of the rat hepatocytes. Within the nucleus, the gold particles appeared to be intensely localized in the areas of euchromatin and nucleolus. Within the cytoplasm, gold particles did not seem to adhere to mitochondria or lysosomes, but were freely distributed. However, rough endoplasmic reticulum was the obvious compartment on which the gold particles were localized. Time course of MT immuno re activity revealed that distribution of gold particles in hepatocytes increased gradually by 24 h, and decreased at 48 h after PH. Briefly, PH resulted in the sharpest increase in the expression of MT mRNA at 8 h and in the immuno re activity of MT at 24 h, respectively. It is suggested that the increase of MT mRNA expression, the intensity of immuno re activity and the specific localization of MT may be associated with the compensatory cell proliferation followed by PH.
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KEYWORD
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Partial hepatectomy, Regeneration, Metallothionein, Metallothionein mRNA, Immunocytochemistry
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